DCI Bern offers expert support and training in:

• Cryo-FIB-Milling: Precise site-specific preparation of cellular lamellae for cryo-EM tomography, optimizing sample quality for in-depth structural studies. We are equipped to mill both waffle grids and standard plunge-frozen grids, enhancing flexibility and compatibility with various cryo-EM sample preparation methods.
• Cryo-CLEM: Bridging light and electron microscopy with cryo-CLEM, combining dynamic imaging of cellular structures with high-resolution cryo-EM. This approach allows researchers to localize specific regions of interest in cells with fluorescence before capturing high-resolution structural details with cryo-EM.
• Cryo-EM Tomography: 3D imaging of cells and large molecular complexes for detailed visualization of cellular architecture. Cryo-EM tomography provides nanoscale insights into the arrangement of biomolecules in their native environment, supporting advanced structural studies of cell morphology and function.
• Cryo-Ultramicrotomy: Precision sectioning at cryogenic temperatures using the Leica EM UC6 Cryo Ultramicrotome, ideal for preparing ultra-thin sections for tomography and ultrastructural analysis.
• Single Particle Analysis: High-resolution imaging of isolated macromolecular complexes using single particle analysis on the Titan Krios G4, ideal for structural studies of proteins and other biomolecules. By capturing biomolecules in multiple orientations, this technique uncovers precise structural details, delivering clarity down to near-atomic resolution.
• Training and Instrument Access: Extensive user training to ensure independence in cryo-EM, cryo-CLEM, and FIB-milling workflows. We provide personalized training sessions on each instrument and workflow, equipping researchers with the skills and confidence to carry out their studies independently.

Customized Protocols and Collaborative Projects

In addition, DCI Bern provides protocol development and collaborative support tailored to complex experimental requirements, ensuring that each project achieves optimal results.

Please reach out to discuss services, pricing, or specific project needs.