Cryo-Correlative Light and Electron Microscope that bridges light and electron microscopy, allowing for dynamic imaging and precise localization studies in combination with high-resolution cryo-EM. Ideal for workflows that combine cellular context with structural detail.
Leica THUNDER Imager EM Cryo CLEM
Instrument Parameters
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CameraLeica DFC9000 GT CMOS camera, high sensitivity, fast frame rate
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Light SourceHXP (High-Pressure Xenon) lamp for broad-spectrum fluorescence excitation
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ObjectivesLeica HCX PL APO 50× (NA 0.90)
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FiltersDAPI (~358 nm excitation / ~461 nm emission)
GFP (~488 nm excitation / ~509 nm emission)
RFP (~555 nm excitation / ~584 nm emission) -
Cryo CompatibilityDedicated cryo stage and sample holders to maintain vitrified conditions
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Imaging FeaturesFast image acquisition to minimize devitrification risk
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SoftwareLeica Application Suite X (LAS X) for acquisition and analysis